Method of analyses

Recommended level


Serum’s lipids1)

Total cholesterol

Enzymatic photometry, The Architect c8200 analyzer (Abbott Diagnostics, Abbot Park, IL, USA)

<5.0 mmol/l (200 mg/dL)

HDL cholesterol

>1.0 mmol/l (men) and >1.3 (women)

Triglyceride content

<1.7 mmol/l (151 mg/dL)

LDL cholesterol

By computationally formula of Friedewalds with total and HDL-cholesterol content

<3.0 mmol/l (100 mg/dL)

Blood glucose1)

Fasting glucose (0 h)

Entzymatic photometry (regulatory enzyme as hexokinase), the Architect c8200 analyzer (Abbot Diagnostics, Abbot Park, IL, USA)

≤6.0 mmol/l (normal); 6.1 - 6.9 mmol/l (increased fasting glucose (IFG)); ≥7.0 mmol/l (diabetes)

2 hours after challenge (2 h)

<7.8 mmol/l (normal); 7.8 - 11.0 mmol/l (impaired glucose tolerance (IGT); >11.0 mmol/l (diabetes)

Measurements at baseline (T0) and the end of the intervention (T3)

Blood pressure (systolic/diastolic)1)

Oscillometric, Omron M3 and Microlite BP A100: in a sitting position, righ arm, after 15 minutes of rest and two measures at each time, which of better one remained in force.

≤130/85 mmHg (normal); 130/85 mmHg - 140/90 mmHg (satisfactory, but needs checking once in a year); >140/90 mmHg (at raised level).

Body Mass Index (BMI)1)

18.5 - 24.99 kg/m2 (normal)

Fat percentage2)

Body composition meter (Omron BF 500). Weight was measured with light indoor clothes and height without the shoes.

8 - 21.9 % (men, 18 - 59 y); 21 - 33.9 (women, 18 - 59 y)

Based on electrical conductivity. Muscle tissue conducts electricity better and faste tahn adiposity tissue. Omron BF-500 meter takes note the total body fat content.

Waist circumference3)

Tape measure from midway between the lowest rib and iliac crest.

≤94 cm (men) and ≤80 cm (women) (normal); >102 cm (men) and >88 cm (women) (significant health risk)

Reveals on amount of visceral fat and fat in area of abdominal cavity.