| cardiac biochemical markers | Methods (Spectrophotometry) | Wave length (nm) |
| Alanine aminotransferase (ALT) | Absorption kinetics (Disappearance of NADH) | 340 |
| Aspartate aminotransferase (AST) | Absorption kinetics (Disappearance of NADH) | 340 |
| Creatine phosphokinase (CPK) | Absorption kinetics (Formation of NADH/H+) | 340 |
| Creatine phosphokinase (CPKMB) | Absorption kinetics (Formation of NADH/H+) | 340 |
| Lactate dehydrogenase (LDH) | Absorption kinetics (Disappearance of NADH) | 340 |