Primer use

Primer name

Primer sequence

1st round 3'RACE

RC146-R1

5'-TCCCTCTGCATCAAGACACTTACCCATT-3’

2nd round 3'RACE

RC146-R2

5'-TTGAGCCCAGGAGTTTGAGACCAGC-3’

1st round 5'RACE

PMCAF1

5'-CGCCTGACCATCCTCCCTGCTCT-3’

2nd round 5'RACE

PMCAF2

5'-GGGGAGGCGAGTTCTCTGCCTTAA-3’

Realtime PCR forward

β-actin-F

5'-CGGATAACACAAGGAAAGGAAAC-3’

Realtime PCR reverse

β-actin-R

5'-ATGGATGGAAACACGGCTCT-3’

Realtime PCR forward

RT-PMCAF

5'-GGTGTATGAAGACGGACCAAAAC-3’

Realtime PCR reverse

RT-PMCAR

5'-GCCATGAAGTCAACGTAGAGGA-3’