Primers

Sequence 5’-3’

Usage

IPT1-PF

CTCCGACAAAATGCAAGTCTAC

Amplification of CmIPT1 middle fragment

IPT1-PR

TTCACAATCT TTACGCTTGG

Amplification of CmIPT1 middle fragment

IPT1-5’-R1

CGACCCGAGACTCCGAAAATCCGAG

Amplification of CmIPT1 5’ end

IPT1-5’-R2

GGGTCGGGTCAATGGCTCCGAGAAG

Amplification of CmIPT1 5’ end

IPT1-3’-F1

CACGTGTCAGTTGGCGAAAAGACAAATAGG

Amplification of CmIPT1 3’ end

IPT1-3’-F2

GAGTGGATGCCACCGAGGCGTTTAA

Amplification of CmIPT1 3’ end

IPT1-RTPF

CGCCAAGTTCTTTCCCGAGTATCC

qRT-PCR for CmIPT1

IPT1-RTPR

ATGCCAAGGTCTCTGAGCTTCTGC

qRT-PCR for CmIPT1

18S-F

CTCATGGGATGTGGCTTCTT

Reference gene in Chrysanthemum

18S-R

GCGTTCAAAAACTCGATGGT

Reference gene in Chrysanthemum

Tubulin-F

GAGCCTTACAACGCTACTCTGTCTGTC

Reference gene in Arabidopsis

Tubulin-R

ACACCAGACATAGTAGCAGAAATCAAG

Reference gene in Arabidopsis