| Trial | % A | % B | Retention Time (min.) | |
| for 2a | for 2b | |||
| 1 | 95 | 5 | 4.155 | 5.142 |
| 2 | 80 | 20 | 4.145 | 5.12 |
| 3 | 70 | 30 | 4.109 | 5.181 |
| 4 | 60 | 40 | 3.936 | 5.046 |