| Adapters/primers | Sequence (5’-3’) |
| Adapters |
|
| HpaII/MspI-adapter I | 5’-CGACTCAGGACTCAT-3’ |
| HpaII/MspI-adapter II | 5’-GACGATGAGTCCTGAGT-3’ |
| EcoRI-adapter I | 5’-CTCGTAGACTGCGTACC-3’ |
| EcoRI-adapter II | 5’-AATTGGTACGCAGTCTAC-3’ |
| Mse1-adapter I | 5’-GACGATGAGTCCTGAG-3’ |
| Mse1-adapter II | 5’-TACTCAGGACTCAT-3’ |
| Pre-amplification primers |
|
| HpaII/MspI-pre | 5’-GATGAGTCCTGAGTCGG-3’ |
| Eco-pre | 5’-GACTGCGTACCAATTCA-3’ |
| Mes-pre | 5’-GATGAGTCCTGAGTAAC-3’ |
| Selective amplification primers |
|
| HpaII/MspI primers |
|
| HM-CTA | 5’-GATGAGTCCTGAGTCGGCTA-3’ |
| HM-CAG | 5’-GATGAGTCCTGAGTCGGCAG-3’ |
| HM-CTT | 5’-GATGAGTCCTGAGTCGGCTT-3’ |
| EcoRI-primers |
|
| EcoR-ACG | 5’-GACTGCGTACCAATTCACG-3’ |
| EcoR-AAC | 5’-GACTGCGTACCAATTCAAC-3’ |
| EcoR-ACA | 5’-GACTGCGTACCAATTCACA-3’ |
| EcoR-ACT | 5’-GACTGCGTACCAATTCACT-3’ |
| EcoR-AAG | 5’-GACTGCGTACCAATTCAAG-3’ |
| EcoR-ACC | 5’-GACTGCGTACCAATTCACC-3’ |
| MseI-primers |
|
| MseI-CTA | 5’-GATGAGTCCTGAGTAACTA-3’ |
| MseI-CTT | 5’-GATGAGTCCTGAGTAACTT-3’ |
| MseI-CAG | 5’-GATGAGTCCTGAGTAACAG-3’ |