| Media | Combination of phytohormones concentration ( mg/L) | Percentage response of callus induction | Number of times subcultured | Observation | ||
| BAP | NAA | IBA | ||||
| MS | 0.5 | 1.0 | 0.1 | 10 | 1 | Callus |
| Vermicompost extract | 0.5 | 1.0 | 0.1 | 60 | 4 | Callus |
| Vermicompost only | - | - | - | 70 | 4 | Callus |
| Vermicompost extract only | - | - | - | 100 | 4 | Callus |
| Vermicompost + coelomic fluid (3:1) | - | - | - | 98 | 4 | Callus |
| Vermicompost extract (explants given coelomic fluid spray) | - | - | - | 100 | 4 | Callus |