| Gene ID | Primer Name | Sequence (5’-3’) | Tm (˚C) |
| Gh_A08G0457 | FLA7-F | GTTGCAATGAGCACAAGCCCGACACCG | 65.8 |
| FLA7-R | GTACGAAAATCGTAATGCCTTCAACCG | 58.2 | |
| Gh_D02G0381 | LTP-F | TTTACCACCCCATTCAAATGATACCC | 57.2 |
| LTP-R | TTAGGAGATGGTGGTCCAAGTTGAAG | 58.3 | |
| Gh_A11G0727 | PME2-F | CTTCGAAGCGGTCCGGAAGTGGAAGG | 64.7 |
| PME2-R | CACTGCCTGATGGTTAGTAGGACCAG | 60.4 | |
| Gh_D08G1266 | MYB46-F | CTGATATCAGAAGAGGGAGATTTACTC | 54.2 |
| MYB46-R | AGTACTTGGTGGAGGAGCAGTGCTTG | 62.3 | |
| Gh_D09G2166 | CESA-G3-F | TCAACAACATTCCACGACGGGTGACG | 62.9 |
| CESA-G3-R | GTCGCAGTCAAGCATTAGAATATATGG | 55.7 | |
| Gh_D02G0079 | UGT74F2-F | GATGAAGGCAGATTCTTTGAAGTTAGG | 55.9 |
| UGT74F2-R | TATGTAATTCACAGCGCACGCCTGCG | 63.3 | |
| Gh_D06G0375 | 6-PGL2-F | GGAAAACGTAGTCCCTAAGCGACACC | 60.9 |
| 6-PGL2-R | GACAGTCGGTTGATCGGGAAACCTG | 61.3 | |
| Gh_A11G0400 | PER72-F | CTTCCAAGACAGTTGGAGGAGGATAC | 58.4 |
| PER72-R | AGCTGAGTTCCTGTTTGGATTCGACC | 60.7 | |
| Gh_A08G0780 | E3-F | GATTCCTTGTTCTCATGCCAAGATCC | 57.8 |
| E3-R | TAACTATTGGTTTTGCTCCAGGGAGC | 59.0 | |
| Gh_A01G0064 | B3-F | ACAGAGGATAAGCTCTCTCAAGCAGG | 59.6 |
| B3-R | TGCTCGTTCCATTACAGCCAATTTGG | 59.9 | |
| Gh_A04G0106 | ERF3-F | CTCGAATACTACCGCTTTTCCGATCG | 59.0 |
| ERF3-R | GATGATATTTCCGGTCCGACACAACC | 59.4 | |
| Gh_A04G0473 | RPS5-F | GCCTCACTGCAAGACCCAACCAAGTC | 63.6 |
| RPS5-R | GCACTGAGCCTTTCTAAAACGCTTAAC | 58.4 | |
| 18S rRNA-F | GGAATTGACGGAAGGGCACCACCAGGC | 78.0 | |
| 18S rRNA-R | GGACATCTAAGGGCATCACAGACCTG | 68.0 |