| Microbial Parameters | Unit | Used Agar | Incubation Period and Temperature | Methods |
| FC | CFU/100ml | MFC | 24 hours and 44.5˚C water bath | Membrane Filtration (MF) technique |
| TC | M Endo | 24 hours and 37˚C | ||
| E. coli | M TEC | 35˚C for two hours after two hours 44.5˚C for 24 hours water bath |