Gene ID | Type | Sequence homology | Genbank acc.no. | Primer Sequences (5'-3') | Size (bp) | Tm (˚C) | PCR efficiency (%) |
Act | Ref† | β-Actin | EU664318 | TAGGTGCCGAGCGTTTCC CCGGGGAACATAGTCGTACC | 175 | 79.2 | 95.7 |
ADF | Ref† | Actin depolymerizing factor | JZ818469 | GCATCTGGTATGGCAGTCC GCACTCATCAGCAGGAAGG | 183 | 81.4 | 91.0 |
APT | Ref† | Adenine phosphoribosyltransferase | JZ818470 | GGAGCTGTTGAAGCTGGTG CACGACCCTTCAGTTCTGG | 154 | 81.8 | 96.7 |
ATPase | Ref‡ | Vacuolar H+- ATPase A subunit | JZ818471 | CTACGAACGTGCTGGGAAAG GAGGGTTGCAGATGTCACG | 124 | 80.2 | 91.4 |
CAC | Ref† | Clathrin adaptor complex | JZ818472 | AGCCGGGCCTCTTAGTATGAC CCCATCGATACGGATTATGAGC | 113 | 76.5 | 97.0 |
COP | Ref‡ | Coatamer delta subunit | JZ818473 | GGTGAGAATCAAGCCGTCTC GTTGGACCAGTGGGGAAAG | 116 | 77.8 | 95.3 |
COMT | Ref‡ | Caffeic acid 3-0-methyltransferase | JZ818474 | ATACTTCCGGTGGCTCCAG GCACCTTTGGCAAGATCCTC | 131 | 81.0 | 94.3 |
eEF-1α | Ref‡ | Eukaryotic elongation factor 1-alpha | JZ818475 | GAGCCAAAGAGACCCACAGAC TCAGTGAGAGCCTCGTGGT | 194 | 82.2 | 93.2 |
eIF-2 | Ref‡ | Eukaryotic translation initiation factor 2 | JZ818476 | GGTGCTGGGTCATCAAAGG GCTCTGGGTCCTGGACAAC | 162 | 78.5 | 94.2 |
eIF-5 | Ref† | Eukaryotic translation initiation factor 5 | JZ818477 | ATGCACTGGAGGAAGAGCAC TCCTCCGACTCTGACTCTGC | 133 | 79.5 | 97.2 |
GAPDH | Ref† | Glyceraldehyde 3-P Dehydrogenase | JZ818478 | CTGGAGAGGTGGAAGAGCTG GGTTGGGACACGGAATGAC | 127 | 82.1 | 97.8 |
ITR | Ref‡ | Inositol Transporter | JZ818479 | TGGTCTTGGTGTAGGGATGG GGAAAGGAACTGTCCACCAG | 127 | 79.6 | 98.8 |
Pro1 | Ref‡ | Profilin 1 | JZ818480 | CCTCGAATGACAGCTCCAG TCCTCGGTGTAGACGGTAGC | 181 | 80.6 | 97.5 |
Rer1 | Ref‡ | Retention in endoplasmic reticulum 1 protein | JZ818481 | GCCTTCTGATGGTGGACCT GGCCAGAAGACAGGAACATC | 165 | 78.9 | 98.0 |
18S rRNA | Ref† | 18S ribosomal RNA | JZ818482 | GGGCTCGAAGACGATCAG AGCCTTGCGACCATACTCC | 145 | 82.3 | 97.8 |
RPL4 | Ref† | Ribosomal protein L4 | JZ818483 | GGATGGCTTTGCTTGCTG CTTTCCCTGCAGCCTTGA | 114 | 80.0 | 99.7 |
Tub | Ref† | α-tubulin | JZ818484 | CAGCCTCCTTCAGTTGTGC CTTCTTCCATGCCCTCACC | 175 | 82.6 | 96.2 |
UBQ-2 | Ref‡ | Ubiquitin 2 | JZ818485 | GGACTCAAGGTGGCCAAAC GCCTAAGCCAGTGGGTGTCT | 197 | 82.5 | 93.0 |
UBQ-5 | Ref† | Ubiquitin-like protein 5 | JZ818486 | GAAGGTTCGCGTGAAGTGC CACCGAGAGTGATGTGATCC | 140 | 82.1 | 92.0 |
UBL-2a | Ref† | Ubiquitin protein ligase 2a | JZ818487 | CCAAACCCAAACTCACCAG AGCAGTCCAACTCTGCTCAAC | 108 | 80.5 | 95.9 |
Unknown | Ref‡ | No hit | JZ818488 | ATGAGCCTTCGTCGTTGC GGAGCCAATCTAGCTGGAAC | 169 | 79.8 | 99.0 |
|
|
|
|
|
|
|
|
ProDh | GOI | Proline dehydrogenase | JZ818489 | GGCTGCTGCAAAAGCACA GCCCTTCTCAAGAGGTATGG | 180 | 79.6 | 98.4 |
SPS | GOI | Sucrose phosphate synthase | JZ818490 | TCCCAAGCCCTCAGATACC CTGCTTCCGACTCCCTTCA | 146 | 80.6 | 96.4 |
SuSy | GOI | Sucrose synthase | JZ818491 | CCGATTGACATCCTTCTACCC GTCCTTTGACTCCTTCCTCCT | 235 | 82.6 | 93.0 |