Antimitotic agents

Number of metaphase^*

1

Cold water

7

2

Cycloheximide (6.25 ppm)

8

3

Colchicine (0.1%)

8

4

Colchicine (0.5%)

5

5

Esculin (0.04%)

8

6

9-Aminoacridine hydrochloride (0.05%) + 8-hydroxyquinoline (0.002M)

5

7

9-Aminoacridine hydrochloride (0.05%) + paradichlorobenzene (saturated solution)

3

8

Hydroxyurea (2.5 mM)

13

9

Paradichlorobenzene (saturated solution)

11

10

8-Hydroxyquinoline (0.02 M)

26

11

8-Hydroxyquinoline (300 ppm) + cycloheximide (6.25 ppm)

11