Sample | Technique |
Maternal serum | IgG/IgM, ELISA, Western blot, immunofluorescence |
Maternal serum | In situ hybridization |
Maternal serum | AFP (Elevated prior to detction of hydrops) |
Maternal serum | AFP (Elevated) |
Maternal serum | PCR |
Maternal serum | IgG antobodies against viral non-stuctural protein NS1 |
Amniotic fluid | PCR |
Amniotic fluid | PCR, southern blot, chemiluminescence |
Amniotic fluid | Enzymatic amplification of parvovirus B19 (segment) |
Fetal blood | PCR, dot-blot hybridization, in situ hybridization |
Fetal blood | Enzymatic amplification of parvovirus B19 (segment) |
Fetal serum | PCR |
Fetal heart tissue | Microscopy: VP1 and VP2 viral particles |
Fetal tissues | Microscopy: histology was found to be as sensitive as PCR |