Sample

Technique

Maternal serum

IgG/IgM, ELISA, Western blot, immunofluorescence

Maternal serum

In situ hybridization

Maternal serum

AFP (Elevated prior to detction of hydrops)

Maternal serum

AFP (Elevated)

Maternal serum

PCR

Maternal serum

IgG antobodies against viral non-stuctural protein NS1

Amniotic fluid

PCR

Amniotic fluid

PCR, southern blot, chemiluminescence

Amniotic fluid

Enzymatic amplification of parvovirus B19 (segment)

Fetal blood

PCR, dot-blot hybridization, in situ hybridization

Fetal blood

Enzymatic amplification of parvovirus B19 (segment)

Fetal serum

PCR

Fetal heart tissue

Microscopy: VP1 and VP2 viral particles

Fetal tissues

Microscopy: histology was found to be as sensitive as PCR