Primer name

Primer sequence

Product size (bp)

Reaction condition

LGR5

CTCCCAGGTCTGGTGTGTTG

GAGGTCTAGGTAGGAGGTGAAG

149

1) 95˚C 30 sec 1 cycle

2) 95˚C 3 sec

β-actin

F GAGCTACGAGCTGCCTGAC

R GTAGTTTCGTGGATGCCAC

120

60˚C 30 sec 40 cycles