| Combination code | EcoRI Primer Sequence (E) | MseI Primer Sequence (M) |
| E38/M32 | 5’-GACTGCGTACCAATTCACT-3’ | 5’-GATGAGTCCTGAGTAACAG-3’ |
| E39/M32 | 5’-GACTGCGTACCAATTCAGA-3’ | 5’-GATGAGTCCTGAGTAACAG-3’ |
| E39/M34 | 5’-GACTGCGTACCAATTCAGA-3’ | 5’-GATGAGTCCTGAGTAACTA-3’ |
| E40/M45 | 5’-GACTGCGTACCAATTCAGC-3’ | 5’-GATGAGTCCTGAGTAACTG-3’ |
| E40/M36 | 5’-GACTGCGTACCAATTCAGC-3’ | 5’-GATGAGTCCTGAGTAACGT-3’ |
| E/45/M35 | 5’-GACTGCGTACCAATTCATG-3’ | 5’-GATGAGTCCTGAGTAACTG-3’ |