Locus

Primer sequences (5'?3')

Repeat

motif

Ta (°C)^a

Size (bp)

Total No. alleles

U. brizantha

U. decumbens

diploid

U. decumbens

tetraploid

U. humidicola

U. ruziziensis

PIC

No. alleles

No. alleles

No. alleles

No. alleles

No. alleles

DecSSR181

F: AGTTGGTGTTGGCAATAACTCTG

R: CTTCTTGGCAGTTGAAGCATCT

CGG(3*5)

60

180 - 210

5

4

1

2

2

3

0.60

DecSSR184

F: CCGAACTTGAGTTGTTATTTCCA

R: AACTGATGGTAACTCTTGGAGCA

TGG(3*5)

60

120 - 140

2

1

1

1

2

1

0.12

DecSSR186

F: CAGGAACGGGGAGACGAG

R: CTCACCAACGTCGGCCTC

CGG(3*5)

60

80 - 90

1

1

1

1

x^b

1

x

DecSSR187

F: GGTGCGTACAACAACTACTGATCT

R: CTGTGCACGCTCGCTGAC

GCT(3*5)

62

150 - 170

5

2

5

2

x

2

0.66

DecSSR188

F: GTGTGCCTCTGAGACCCAGT

R: CCCAGCTCAGCCAGTACTTC

GGA (3*5)

62

120 - 140

4

1

1

3

1

1

0.31

DecSSR194

F: CTCCGCCTTGCAGTACCTC

R: TGCAGTACAACAACTACCACCTC

GCT(3*5)

60

110 - 120

2

2

2

2

x

2

0.50

DecSSR195

F: GTCCCGGTGGTGGTGTCC

R: GTTCCACCTGATCTCGCTCTC

GGA(3*5)

62

90 - 100

4

2

1

3

x

2

0.56

DecSSR199

F: ATTCAGATGCATTTTTCTTCGTC

R: AGCAGCGTATAATAAGCAAGCAC

TCA(3*5)

60

140 - 160

2

x

2

2

x

2

0.46

DecSSR202

F: CTGCTTCTTATAGATCCGACCAC

R: TCTTTAGGAGAGGGATCGAGATT

TCG(3*5)

60

110 - 150

7

1

4

2

1

4

0.81

DecSSR203

F: GTGATGACGGATGCGGTT

R: ACTATCCGACTTCGCCCAC

GCG(3*5)

62

110 - 100

4

3

2

4

x

1

0.66

DecSSR205

F: GAAATCAGAGATGCCCAGACC

R: AGCGAGATCACCACGGAG

CCG(3*5)

62

90 - 110

2

1

2

1

x

1

0.14

DecSSR206

F: GGTGGTACCCGGAGTTAGAGTT

R: GCTACCACTACGACCAGGACTC

GCC (3*5)

62

100 - 110

3

1

1

2

1

1

0.24

DecSSR211

F: GTATTAATTCACTGGGTGTCGCT

R: CCATCACCTCCATCATAGGC

TTG(3*5)

60

130 - 150

4

1

1

1

2

2

0.56

DecSSR212

F: GCCGTATCTCCTTCTCCCAC

R: CACTACCCCTCCCTCCTCTC

GAG(3*5)

62

110 - 140

13

11

5

5

3

4

0.84

DecSSR213

F: CAGAGGAAAGGAAGCAGCAG

R: GGCATCACCACCGTCTGG

CAG(3*5)

62

110 - 130

8

7

2

4

x

3

0.81

DecSSR214

F: AGGAACTGGTGCTCCTTCTTC

R: TCCTACAAGTACACAATCCCACC

GGC(3*5)

60

120 - 160

7

5

3

5

1

5

0.77

DecSSR217

F: TCTGCAAACTGAGGTTCTTCTTC

R: TTGCCAAACGAGACCATAGAGTA

TTC (3*5)

55

260 - 310

4

1

1

3

x

1

0.41

DecSSR218

F: TATCTCTATCTGGGGATTGGAGC

R: AAACAAAACCAAATCGCGG

GCG(3*6)

60

140 - 160

6

3

3

4

3

2

0.80

DecSSR221

F: ATCGACACTACTCTCGGCATAAC

R: GTATGTGGTGGAGGTAGTTGGG

TCG(3*5)

60

120-140

3

1

1

1

2

2

0.55

DecSSR222

F: GGTCATCACCGACTGGTTATTTA

R: ACTTGACAAATCTCTGTCCCGTA

GGC(3*5)

55

130 - 140

5

4

1

3

2

4

0.63

DecSSR224

F: AAGACGAAGCGAGCGTTG

R: CTTCCTCTCCTGGTCGTCCT

CGG(3*5)

62

90 - 100

2

1

2

1

1

2

0.33

DecSSR228

F: GTCGTCGAGCTTCCCGAG

R: CTGCTAGAGACGCGGAGC

GCC(3*5)

60

100 - 110

3

2

1

2

1

2

0.51