No.

Primer name

EST/Contig ID

Repeat motif

Primer sequence (5'-3')

Ta (˚C)

Product size

No. of alleles

PIC

30

KSL-1

CLSS10849

(CAA)10

F: CACCACTCCATTTCATCCCA

R: GCTCATTCCCAAACCCAGAT

55

162 - 171

3

0.197

31

KSL-37

CLSM1424

(AGA)15

F: TCTCTTGCTCCAATACCCGA

R: GTATCGGGCTCATGTCCCTT

55

125 - 155

6

0.666

32

KSL-51

CLSZ1624

(ATG)10

F: CCCCTACCACCACCAAAGTC

R: TACCAAATGACATGCACCCC

55

184 - 205

4

0.461

33

KSL-271

CLSS8197

(ATG)12

F: ACAAAGGCAAGATTGGGTCA

R: GCGGATATGCAGCCATAACA

55

238 - 250

3

0.505

34

KSL-316

QGC8F02

(AAT)11

F: CGCAGCCTTCAAAACTACCA

R: AGCAACTGAAATCCAACCCC

55

272 - 281

2

0.461

35

KSL-317

QGC8E09

(ATG)11

F: TGTGGATCTGAATGGGCATC

R: TGCAAGAATGTTGGCTTCCT

55

248 - 251

2

0.496

36

KSL-357

CLSS10992

(TGA)14

F: GCAGCAACAAGAAACCCAAA

R: GCCCCAACATCATCATCATC

55

255 - 283

2

0.356

37

KSL-87

CLSY5704

(CT)17

F: GCGGGATCGATACTTACCCT

R: ATCATCGACGGGCTTTTCTT

55

256 - 272

7

0.614

38

KSL-173

CLSM444

(CT)14

F: ATAGTCACGACTCACGCCCA

R: CCATTTTCCTCTTTCTGCGA

55

155 - 165

4

0.608

39

KSL-245

CLSM513

(AG)16

F: CTTCACCTCCGGAATCCTGT

R: GAGGCACGACTGCCATTTAG

55

269 - 283

4

0.138

40

KSLC-4

A35

(GGT)5

F: TGGGGGAACATCACAAACAC

R: ACTTCCGACCACCAATAGGG

55

196 - 199

2

0.328

41

KSLC-30

B14

(CA)6

F: GGGGCCTCTATCCACTCTCA

R: AAAGTCCAGCCATCTCTGCC

55

162 - 164

2

0.437

42

KSLC-322

L146

(TTATA)4

F: CTCCTCCGGGAAACTATGGA

R: TCTCCAACACAACACCCACC

55

290 - 300

3

0.349

43

KSLC-443

R439

(GAAGAG)4

F: GACGATGACGACGTGGAAAG

R: CCTCCAGCTGCAAACCAGTA

55

262 - 271

2

0.063

44

KSL-7

CLSS10499

(TCT)12

F: TGCTCAATCTCGAGCTTATCCT

R: ATGTGCCCACAAGGAAGACA

55

378 - 396

3

0.505

45

KSL-26

CLSM14994

(TC)16

F: GGGCTTTCTCTCCTTTCCTTT

R: AATTTGGATCCTGTCGAGGG

55

300 - 319

8

0.743

46

KSL-32

CLSM14764

(CT)14

F: CGGGGAGCATTTAGTGTGTG

R: AATTTGGGGTCCGATTTGAG

55

210 - 218

5

0.610

47

KSL-43

CLSM1373

(ATC)9(TTC)8

F: GACGCAAACCTTTACCAGCA

R: TCATTCCATTCCATTGGGTG

55

269 - 281

3

0.501

48

KSL-44

CLSM13555

(TCACCA)4tcat (CATCAC)5catcg (CCATCA)5

F: CGATTCCTTCACCTCCACCT

R: CTGTCAATCGTGCCAGTTCC

55

229 - 246

3

0.510

49

KSL-75

CLSY7906

(TC)6tt(TC)6

F: AGAGGCTTTCTACGCCAACC

R: TGAGGAGGGGGAAGTTCATC

55

205 - 207

2

0.444

50

KSL-83

CLSY6646

(AG)6tgtgt(GA) 7aa(GT)6

F: GCGGAGCTTCTTTCTCACCT

R: GGAAAGAAAACGCATTTCGAG

55

249 - 287

2

0.315

51

KSL-92

CLSY517

(CT)20

F: GGTCTCTTTCCTCTGCCCTG

R: TCGCGTTCTGAAGTAGCCAT

55

188 - 196

5

0.735

52

KSL-97

CLSY4815

(CT)11

F: CGCAGAAAAGGGATCAGACA

R: TCAGAGACACTGCAAAAGGGA

55

223 - 232

3

0.616

53

KSL-102

CLSY4549

(TGA)5tgt(TGA)6

F: AAGCCCCAATGACGAATCTC

R: TGTCAATAGCAATGGCGAAA

55

313 - 320

3

0.121

54

KSL-115

CLSM11208

(CT)11

F: CATTGCACTCCGTCATCTCC

R: GGGTTGATTCCGAAAGTTCC

55

210 - 212

2

0.477

55

KSL-119

CLSM10279

(TC)16

F: TTCGACTCGTCTTCGACGC

R: CGATGTCACACCACCCATCT

55

271 - 279

4

0.642

56

KSL-123

CLSL2393

(ATC)13

F: ATTGTAACTTCTGCGGGCCT

R: GCCTCACATGTTCTTCCCCT

55

336 - 360

4

0.511

57

KSL-137

CLSZ3622

(TGA)9caatgatgaaaaagatgatgcagaggaggcagatgatgatgctggagatgaagatttctcaggagaagaagggggagag(GAT)6gaagaagaccctagtgaagatcctaaggcaaatggtaatcaagacgac(GAT)7gacgacgac(GAT)6

F: TTCTCTGAGCTTCACAAGAGGG

R: TCATCACCATCATCATTTCCC

55

281 - 298

4

0.666

58

KSL-152

CLSM12854

(GAC)5gatgagc (AAG)8

F: CGAGGTCGAAGAAGAGGAGG

R: TCCCATTAGGATCTCTGCCC

55

268 - 284

2

0.063

Total

176

24.63

Mean

3.03

0.425+