No.

Primer name

EST/Contig ID

Repeat motif

Primer sequence (5'-3')

Ta (˚C)

Product sizes

No. of alleles

PIC

1

SML-001

QGC15N13

(CATGAT)6

F: CCATGGATCCTGTGTGAAGA

R: CACCATGTTCCACTTCCACTT

55

176 - 198

4

0.581

2

SML-002

QGH4c05

(TTC)17

F: GTGATTGCATGCCAAATGAA

R: TTAGTAGCCCGCATGCTTTT

55

204 - 225

4

0.310

3

SML-003

QGA14A20

(GTTTT)5

F: CGGGCTGGTTTTGATTTTTA

R: TGTCAAATCGTCACGTGGTT

55

114 - 119

2

0.418

4

SML-007

QGG10L02

(TCACCA)19

F: ACACTTGCCGATTCCTTCAC

R: ACCCGTGTTGAAAATGGAGA

55

184 - 202

3

0.519

5

SML-013

Cntg-4755

(GAA)14…(CTG)5

F: TCCCATGATGGAGAGACTCA

R: CCCAAAAGGGAATAGCAACC

55

265 - 276

3

0.434

6

SML-015

Cntg-1438

(TGTTA)16

F: TTGAGGAGGGCATTTACGTC

R: GAGGCGTATCTCCAAGGTGT

55

254 - 269

3

0.471

7

SML-019

Cntg-1238

(ATATG)5

F: AAGGAGGAAAGTATGGTGAGGA

R: TGAAATGAAGCAACACACGA

55

163 - 168

2

0.375

8

SML-020

Cntg-419

(AATG)6

F: GTGGTCGTGATGATGCTTTG

R: TGCAATCCCTCTTTTCTTCAA

55

223 - 227

2

0.141

9

SML-038

Cntg-4846

(CCA)4

F: ACCTCCTCCGTCACCAAGT

R: TCGCAAATTTTCTTGCCTTT

55

189 - 192

2

0.291

10

SML-039

Cntg-5632

(CCCCTT)2

F: ATTACCCCTGGCCTTATGCT

R: TCGTATCTTGGCTGCTCCAT

55

229 - 235

2

0.444

11

SML-042

Cntg-6454

(CGGA)2 (AGGA)3 AAG (A)11* (GAAAGA)2

F: CATGAAGTGTTTTGGGGTGA

R: GGCCTTTCATTTCTTCCTCA

55

189 - 193

3

0.435

12

SML-043

Cntg-649

(T)14

F: TTCTTTCGCCCATCTGAAAC

R: AAACAGGGGGCTAACGATCT

55

192 - 198

3

0.468

13

SML-045

Cntg-7478

(AAG)9

F: ACAAAACCGTTTCACCCAAA

R: AGCCCTGTCCTCTTCAGGAT

55

220 - 226

3

0.375

14

SML-048

Cntg-7935

(T)12

F: TTTGAGGCAGGTGTTAGCTG

R: GAACAAAGTTACAACGAGAGGAAA

55

114 - 123

3

0.337

15

SML-051

QGC13L24

(TAA)8

F: CTCAAGGCGGCATCTACTTC

R: GACCCCTTGCTTGTTTCAGA

55

237 - 240

2

0.315

16

SML-052

Cntg-2202

(CAT)5

F: CTGTAGCCGGGAATTGAAGT

R: TGCCCCTAAACAAGACCTACA

55

219 - 228

2

0.022

17

SML-054

Cntg-3649

(TA)3

F: CCATGCCATGCAGTATACCTT

R: AAAAATGACTGCATACTTTGTGAA

55

269 - 271

3

0.529

18

SML-055

Cntg-3666

(TGA)15…(ATG)9

F: CTGCGTGTTTTAAGCCGTTT

R: TCCATAATAATATAATCGCACCAA

55

224 - 239

2

0.378

19

SML-056

QGE4h11

(TTA)14

F: GCCTAGTCCAATTGCTTTGC

R: CAGCTTAACATACTTTTGTTCATTCA

55

183 - 189

2

0.350

20

SML-057

QGB14L18

(GAA)14…(CTG)5

F: TCCCATGATGGAGAGACTCA

R: CCCAAAAGGGAATAGCAACC

55

266 - 278

3

0.437

21

SML-059

Cntg-4454

(TCT)12

F: GATAAAGGCTGGACCGATGA

R: GTTTGGTTTGGTTTGGCAAG

55

177 - 208

3

0.536

22

SML-061

Cntg-3414

(AACA)6

F: GAGTGCTGAGAAAGCCCAAG

R: TAAGCTGCTCTTCCCTCCTG

55

203 - 207

2

0.402

23

SML-021

Cntg-1077

(TA)8

F: TTGGGAGAATTTTCATTTCCA

R: AGTCATCTTTTTCACCCCACA

55

172 - 180

4

0.521

24

SML-022

Cntg-1211

(ATC)13

F: GGGCCTCAAATCCTCTCTG

R: TGTTCTTCCCCTCTTTGGAA

55

314 - 338

4

0.511

25

SML-026

Cntg-2481

(GAA)11

F: GGGTTCTCATTGGCTGACAT

R: TGTCTTCCAACCAAAACATACA

55

172 - 198

3

0.500

26

SML-028

Cntg-2789

(A)15

F: TGATCCAGGCTCTCCAGAAT

R: CACGACCATGAATGATAAGTGC

55

170 - 181

3

0.511

27

SML-032

Cntg-3460

(T)4

F: TTGGATTTGGGGTGATGAAT

R: GCATAGTAATTTGACATTTTGGCATA

55

215 - 216

2

0.465

28

SML-036

Cntg-4454

(TCT)12… (CCAAA)4

F: CTGCTGCTGTTTTGCTCTTG

R: CCTGAGGTGAGGTTGTCAAT

55

214 - 217

2

0.137

29

SML-037

Cntg-4499

(AAC)3

F: TTTTTCCCGATCTTTGCATC

R: AGCGAATCTTTGCTTTTTCG

55

211 - 214

2

0.282