PCR Types

Primers

Sequences

Quantitative PCR (qPCR) for Viral Load on RNA

qPCR

HIV1MG Forward

5’-GCCTCAATAAAGCTTGCCTTGA-3’

HIV1MG Reverse

5’-GGCGCCACTGCTAGAGATTTT-3’

HIV1MG Probe

FAM-5’-AAGTAGTGTGTGCCCGTCTGTTRTKTGACT-3’-BHQ1

PCR for Sequencing

RT-PCR Prot

5’ prot 1

5’-TAATTTTTTAGGGAAGATCTGGCCTTCC-3’

3’ prot 1

5’-GCAAATACTGGAGTATTGTATGGATTTTCAGG-3’

Nested PCR Prot

5’ prot 2

5’-TCAGAGCAGACCAGAGCCAACAGCCCCA-3’

3’ prot 2

5’-AATGCTTTTATTTTTTCTTCTGTCAATGGC-3’

RT-PCR RT

MJ3

5’-AGTAGGACCTACACCTGTCA-3’

MJ4

5’-CTGTTAGTGCTTTGGTTCCTCT-3’

Nested PCR RT

A (35)

5’-TTGGTTGCACTTTAAATTTTCCCATTAGTCCTATT-3’

NE1 (35)

5’-CCTACTAACTTCTGTATGTCATTGACAGTCCAGCT-3’

RT-PCR Alt Prot

5’ eprB

5’-AGAGCTTCAGGTTTGGGG-3’

3’ eprB

5’-GCCATCCATTCCTGGCTT-3’

Nested PCR Atl Prot

5’ prB

5’-GAAGCAGGAGCCGATAGACA-3’

3’ prB

5’-ACTGGTACAGTTTCAATAGG-3’

RT-PCR Atl RT

RT1

5’-CCAAAAGTTAAACAATGGCCATTGACAGA-3’

RT4

5’-AGTTCATAACCCATCCAAAG-3’

Nested PCR Alt RT

RT18

5’-GGAAACCAAAAATGATAGGGGGAATTGGAGG-3’

RT21

5’-CTGTATTTCTGCTATTAAGTCTTTTGATGGG-3’