| Plant growth regulators (PGRs) | Concentrations (mg/L) | Number of explants inoculated | Number of explants survived | Survival rate (%) | Number of explants give rise to callus | Percentage (%) of callus response (±SD) | Color of callus |
| 2,4-D | 0.5 | 15 | 14 | 93.3 | 0 | 0 | N/A |
| 1.0 | 15 | 12 | 80 | 02 | 16.7 ± 1.20 | Whitish | |
| 1.5 | 15 | 15 | 100 | 04 | 26.7 ± 2.05 | Yellowish | |
| 2.0 | 15 | 14 | 93.3 | 06 | 42.9 ± 2.27 | Greenish | |
| 2.5 | 15 | 13 | 86.7 | 03 | 23.1 ± 1.80 | Yellowish | |
| 3.0 | 15 | 15 | 100 | 14 | 93.3 ± 0.50 | Whitish | |
| 3.5 | 15 | 15 | 100 | 13 | 86.7 ± 1.75 | Whitish | |
| 4.0 | 15 | 07 | 46.7 | 01 | 14.3 ± 4.05 | Yellowish | |
| BAP + NAA | 0.5 + 0.5 | 10 | 08 | 80 | 02 | 25 ± 3.21 | Greenish |
| 1.0 + 0.5 | 10 | 09 | 90 | 01 | 11.1 ± 1.60 | Yellowish | |
| 1.0 + 1.0 | 10 | 07 | 70 | 03 | 42.9 ± 3.63 | Greenish | |
| 2,4-D + BAP + NAA | 1.0 + 0.5 + 0.5 | 05 | 05 | 100 | 01 | 20 ± 2.89 | Brownish |
| 1.0 + 1.0 + 0.5 | 05 | 04 | 80 | 01 | 25 ± 2.89 | Whitish | |
| 0.5 + 0.5 + 0.5 | 05 | 04 | 80 | 0 | 0 | N/A |