Strains producing HysA and their hysA expression could be detected by qPCR

Strain

Phenotypic assay

Genotypic assay

Rank

Concentration/OD630 nm

(Mean ± SD)

Fold of change

(Mean ± SD)

Rank

S10

1

938.38 ± 94.22

127.86 ± 3.315

1

Newman

2

397.00 ± 4.24

1

3

S5

3

322.29 ± 52.03

0.631 ± 0.023

4

S8

4

295.50 ± 21.92

0.579 ± 0.028

5

S3

5

273.65 ± 75.87

0.025 ± 0.042

17

S9

6

270.16 ± 64.83

0.326 ± 0.027

8

S7

7

236.37 ± 19.28

0.363 ± 0.041

7

S6

8

219.50 ± 14.85

2.243 ± 0.604

2

S2

9

215.62 ± 23.46

0.264 ± 0.113

9

S20

10

212.16 ± 17.20

0.175 ± 0.004

12

S4

11

201.30 ± 15.99

0.413 ± 0.424

6

S13

12

180.49 ± 9.34

0.190 ± 0.083

11

S1

13

145.31 ± 7.51

0.145 ± 0.050

13

S12

14

135.98 ± 43.94

0.226 ± 0.134

10

S23

15

107.50 ± 17.68

0.005 ± 0.0006

19

S21

16

107.30 ± 10.32

0.036 ± 0.0078

16

S11

17

99.15 ± 12.93

0.001 ± 0.0003

20

S22

18

74.87 ± 10.08

0.038 ± 0.001

15

S19

19

72.65 ± 3.33

0.013 ± 0.002

18

S18

20

52.64 ± 10.42

0.137 ± 0.049

14

Strains producing HysA and their hysA expression could not be detected by qPCR

Strain

Concentration/OD630 nm (Mean ± SD)

S14

66.8 ± 15.77

S15

30.1 ± 6.52

S16

20.2 ± 5.43

S17

9.1 ± 2.22

S24

63 ± 14.34