Fan, Y. et al. 2018

To explore and compare the anti-tumor effect of 6 different herbs (PV being one of them).

Soxhlet extraction method was used with a equal volume mix of ethanol and water, and a petroleum ether-ethyl acetate mix respectively. Cell viability was evaluated by a cell counting kit-8 and MTT assay. Cell apoptosis was analyzed by the annexin V-FITC/PI kit. Cell cycle analysis was done by flow cytometry with PI. DNA fragmentation was detected by DNA gel electrophoresis

hepatoma/ BEL7404, HepG2, HepaRG, Huh-7 cell lines

N/A