Zhao A.G. et al. 2008 | To investigate the gene expression changes due to the use of a Chinese herbal formula, Wei Chang An, in gastric cancer cell line SGC-7901 | The gastric adenocarcinoma cells were grafted onto nude mice, which were divided into 3 groups, with one of them as a control group which received saline. One group received the herbal formula injection; another group received 5-FU. After the mice were sacrificed, cancer samples were taken. The gene expression profiles were measured by using a cDNA microarray and then RT-qPCR. The treatment groups were also compared with TUNEL, and immunochemical methods. | gastric adenocarcinoma | Stat3, RIPX, ROD1, Bcl-2 |
Chen C., Wu G., Zhang M. 2009 | To study the effect of PV extract on the Jurkat human T lymphoma cell line. | The cell proliferation and apoptosis were determined by MTT assay and flow cytometry. DNA fragmentation was observed by gel electrophoresis. Western blotting was used to study the proteomics. | lymphoma/Jurkat cells | Bcl/Bax |
Choi J.H. & Jeong H.G 2009 | To study the effect of PV aqueous extract on lung metastasis of melanoma cells. | In vivo, C57BL/6 mice were used to observe the inhibition effect of PV on the number of lung metastatic colonization. In vitro, luciferase activity assay were used to study the expression level of MMP-9 through the mediation of NF-κB. Wound healing assay was also used to examine cell migration. | melanoma/ HT-1080 cells | MMP-9, NF-κB |
Han E.H. et al. 2009 | To study the immunostimulatory and antitumor activities of PV in murine macrophage RAW 264.7 cells | PV extract was obtained by submerging the spica in hot water for 5h. Cell cytotoxicity was assayed by WST-1 reagent. Production of NO was measured by Griess reagent. Cytokine production was quantified by sandwich immunoassays, RT-qPCR, Western blotting and luciferase activity assay. | RAW 264.7 cells | TNF-α, IL-1β, IL-6, NF-κB, MAPK |
Zhang, M.Z. et al. 2009 | To analyse the proteomics change after treatment by PV | Two dimensional electrophoresis and mass spectrometry were used. Cell proliferation by MTT assay. | Lymphoma/ Raji cells | Multiple proteins were identified |
Zhang M.Z. et al. 2009 | To analyse the proteomics change after treatment by PV | Two dimensional electrophoresis and mass spectrometry were used. Cell proliferation by MTT assay. | Lymphoma/ Jurkat cells | Multiple proteins were identified |
Zhang, M.Z. & Wang X.Q. 2009 | To investigate the effects of the extract of PV combined with chemotherapeutic agents on the proliferation of lymphoma cells | Raji cells were treated with PV extract combined with paclitaxel and adriamycin. Cell viability was tested by MTT assay. The cell cycle and apoptosis were studied by flow cytometry, proteins study by immunochemistry. | Lymphoma/ Raji cells | Survivin, caspase-3 |
Cheng, W.W. et al. 2010 | To investigate the effects on the growth and proliferation of breast cancer MCF-7 cells by 9 kinds of herbs. | 9 kinds of herbs were compared. MTT and trypan-blue staining assay was used. Morphological changes of cells were observed. | Breast cancer/ MCF-7 | N/A |
Choi J.H. et al. 2010 | To examine the inhibitory effects of tumor cell migration by aqueous extract of PV | Both in vivo and In vitro assays were used. Expression levels of MMP-9, NF-KB, ERK1/2, mRNA and transcription activities | Melanoma/ B16-F1 cells, B16-F10/ mice | MMP-9, NF-κB, ERK1/2 |