Route of administration

Number of Injections

Total volume of injection (μl)

Age (weeks)

Special Notes

Percutaneous retro-orbital

2

200

10

Jugular catheter

6

600

10

Injections were continued as long as possible to access duration of catheter patency. Each mice with the catheter costs $117 more than the regular mice. These mice needs to be individually housed which makes it expensive than the other routes

Tail vein

2

200

10

Femoral cut down

2

200

16

Not more than two injections possible through this route.