Route of administration | Number of Injections | Total volume of injection (μl) | Age (weeks) | Special Notes |
Percutaneous retro-orbital | 2 | 200 | 10 |
|
Jugular catheter | 6 | 600 | 10 | Injections were continued as long as possible to access duration of catheter patency. Each mice with the catheter costs $117 more than the regular mice. These mice needs to be individually housed which makes it expensive than the other routes |
Tail vein | 2 | 200 | 10 |
|
Femoral cut down | 2 | 200 | 16 | Not more than two injections possible through this route. |