Straw-in-Straw

Open Straw

Inner 0.25 mL straw

Straw end heat-sealed together with the outside 0.5 mL straw end

Straw end open and separated from the outside 0.5 mL straw

Thawing method

Thaw the straw-in-straw in 37˚C water bath, then cut off heat seals and expel thawed sperm into culture medium

Remove the inner 0.25 mL open straw from the 0.5 mL straw and plunge into culture medium to thaw sperm