| Straw-in-Straw | Open Straw |
Inner 0.25 mL straw | Straw end heat-sealed together with the outside 0.5 mL straw end | Straw end open and separated from the outside 0.5 mL straw |
Thawing method | Thaw the straw-in-straw in 37˚C water bath, then cut off heat seals and expel thawed sperm into culture medium | Remove the inner 0.25 mL open straw from the 0.5 mL straw and plunge into culture medium to thaw sperm |