| Gene and Accession Number | Primer | Amplicon Size |
| IGF-I (NM_214256.1)a |
| 146 bp |
| Forward | 5’-ATGCCCAAGGCTCAGAAGGAAGTA-3’ | |
| Reverse | 5’-AGGTAACTCGTGCAGAGCAAAGGA-3’ | |
| IGF-type-1 R (NM_214172.1)b |
| 105 bp |
| Forward | 5’-CCAGGCCAAAACGACATATGA-3’ | |
| Reverse | 5’-GTGGAAGACGTACAACATTATAACCAA-3’ | |
| pIGFBP2 (AF120326.1)c |
| 142 bp |
| Forward | 5’-CGAGCAGGTTGCAGACAATG-3’ | |
| Reverse | 5’-GAACACAGCCAGCTCCTTCAT-3’ | |
| pIGFBP3 (AF085482.1)d |
| 54 bp |
| Forward | 5’-CATCCACATCCCCAACTG-3’ | |
| Reverse | 5’-CGGCACTGCTTTTTCTTGTAGAA-3’ | |
| pIGFBP5 (U41340.2)e |
| 74 bp |
| Forward | 5’-CGCAAGGGATTCTACAAGAGAAA-3’ | |
| Reverse | 5’-TCCACGCACCAGCAGATG-3’ | |
| GnRHR (NM_214273.1)f |
| 101 bp |
| Forward | 5’-AGCCAACCTGTTGGAGACTCTGAT-3’ | |
| Reverse | 5’-AGCTGAGGACTTTGCAGAGGAACT-3’ | |
| LHβ (NM_214080.1)g |
| 151 bp |
| Forward | 5’-ATGCTCCAGAGACTGCTGTTGT-3’ | |
| Reverse | 5’-TGCTGGTGGTAAAGGTGATGCAGA-3’ | |
| pERα (NM_214220)h |
| 91 bp |
| Forward | 5’-GAATGTTGAAGCACAAGCGCCAGA-3’ | |
| Reverse | 5’-ACCGGGCTGTTCTTCTTAGTGTGT-3’ | |
| pKISS1 (NM_0013464.1)i |
| 89 bp |
| Forward | 5’-GGCAGCTGAGTTCTTTCTTTG-3’ | |
| Reverse | 5’-CGGGCCTGTAGATCTAGGATT-3’ | |
| pKISS1R (NM_001044624.2)j |
| 110 bp |
| Forward | 5’-CAGGGAACTCACTTGTGATCTT-3’ | |
| Reverse | 5’-GCACAGCAGAAACGTCAAATC-3’ | |
| pβ-actin (U07786.1)k |
| 129 bp |
| Forward | 5’-TCGCCGACAGGATGCAGAAGGA-3’ | |
| Reverse | 5’-AGGTGGACAGCGAGGCCAGGAT-3’ |