The HPLC method

The SF method

Concentration ratio ATP/IMP, (μmol/g)

Optical Density Ratio ATP/IMP,

(OD260/OD250)

Frozen meat

in a fresh form

after cooling

in a fresh form

after cooling

1

13.92

0.06

1.11

0.72

2

10.64

0.02

1.10

0.72

3

11.92

0.04

1.10

0.71

4

15.58

0.03

1.10

0.75

5

22.11

0.06

1.08

0.73

x ± s

14.84 ± 4.48*

0.04 ± 0.02*

1.10 ± 0.01**

0.73 ± 0.02**