| Name | Sequence | Annealing temperature (˚C) |
| P1F | CAGTAATAAGAAACAAGTTATTTCAAC | 53 |
| P1R | GAACCGGAAGTGTCAGATTAAAACAC | |
| P2F | GCTACCAAGCTTCAGTAATAAGAAACAAGTTATTTC | 55 |
| P2R | GAGTGCGTCGACGAACCGGAAGTGTCAGATTAAAAC | |
| T7 SP | TAATACGACTCACTATAGGGC | 55 |
| 3’AD SP | AGATGGTGCACGATGCACAG |