| Concentration of amino acid (mg/l) | Eugenol (ppm) | Percentage (%) of Increase compared with mother plants (+) | Percentage (%) of decrease compared with mother plants (−) | |
| Mother plants (leaves) | 41.0 |
|
| |
| Control (callus without amino acid) | 0.0 | 15.0 |
| −63 |
| Proline | 50 | 160.3 | +290 |
|
| 100 | 57.8 | +40 |
| |
| 150 | 0.0 |
| −100 | |
| Glutamine | 50 | 71.8 | +75 |
|
| 100 | 68.0 | +65 |
| |
| 150 | 74.9 | +82 |
| |
| Tryptophan | 50 | 56.0 | +36 |
|
| 100 | 51.0 | +24 |
| |
| 150 | 0.0 |
| −100 | |
| Phenyl alanine | 50 | 68.2 | +66 |
|
| 100 | 57.6 | +40 |
| |
| 150 | 0.0 |
| −100 | |