| Concentration of amino acid (mg/l) | Coumarin (ppm) | Percentage (%) of Increase compared with mother plants (+) | Percentage (%) of decrease compared with mother plants (−) | |
| Mother plants (leaves) | 10 |
|
| |
| Control (callus without amino acid) | 0.0 | 90.2 | +802 |
|
| Proline | 50 | 178.9 | +1689 |
|
| 100 | 121.8 | +1118 |
| |
| 150 | 285.2 | +2752 |
| |
| Glutamine | 50 | 113.2 | +1032 |
|
| 100 | 70.0 | +600 |
| |
| 150 | 41.1 | +311 |
| |
| Tryptophan | 50 | 70.7 | +607 |
|
| 100 | 70.4 | +604 |
| |
| 150 | 88.2 | +782 |
| |
| Phenyl alanine | 50 | 45 | +350 |
|
| 100 | 122.3 | +1123 |
| |
| 150 | 146.8 | +1368 |
| |