Concentration of amino acid (mg/l)

Coumarin (ppm)

Percentage (%) of Increase compared with mother plants (+)

Percentage (%) of decrease compared with mother plants (−)

Mother plants (leaves)

10

Control (callus without amino acid)

0.0

90.2

+802

Proline

50

178.9

+1689

100

121.8

+1118

150

285.2

+2752

Glutamine

50

113.2

+1032

100

70.0

+600

150

41.1

+311

Tryptophan

50

70.7

+607

100

70.4

+604

150

88.2

+782

Phenyl alanine

50

45

+350

100

122.3

+1123

150

146.8

+1368