Concentration of amino acid (mg/l)	Amino acid
Concentration of amino acid (mg/l)	Proline	Glutamine	Tryptophan	Phenyl alanine
Callus fresh weight (mg)
0.0	2548	2548	2548	2548
50	2758	2887	2857	2244
100	2193	2923	2951	1923
150	2112	3065	2709	2045
Mean	2402	2855	2766	2190
No statistically significant differences at P ≤ 0.05
Callus dry weight (mg)
0.0	141	141	141	141
50	159	153	157	139
100	138	156	165	138
150	137	160	158	141
Mean	143	152	155	139
No statistically significant differences at P ≤ 0.05