Colorants | Background intensity of 1 µl colorant | Visibility | Inertness | Storage | N1-RNA 1 copy/µl Hs-RNA 104 copies/µl | Covid-19 patient samples | |||||||
In Water | In MMx | In PCR mix | Ct | IU | Ct | IU | |||||||
N1-FAM | Hs-Cy5 | N1-FAM | Hs-Cy5 | N1-FAM | Hs-Cy5 | N1-FAM | Hs-ROX | ||||||
Tartrazine | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Fast Green | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Sunset Yellow | 1 | 1 | 2 | 1 | 1 | 1 | ND | 1 | 1 | 1 | 1 | ND | ND |
Brilliant Blue | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ND | ND |
Acid Red 33 | 2 | 2 | 2 | 2 | 1 | 1 | ND | 1 | 1 | 1 | 1 | ND | ND |
No colorant | R | R | R | R | R | R | R | R | R | R | R | R | R |