| Nucleotide change | Amino acid change | Location | Sequence of primer pairs (5’ to 3’) and PCR condition | Length of PCR product (bp) |
| 144 G->T | Gln48His | Exon 1 | CTTCTGTGCACGTTGCTGCA CTGGTCCAAGGTCAATTGGT 94˚C 30 s, 52˚C 30 s, 72˚C 60 s for 30 cycles using 1 mM MgCl2 | 313 |
| 1109 C->T | Pro370Leu | Exon 3 | ATACTGCCTAGGCCACTGGA CAATGTCCGTGTAGCCACC 94˚C 30 s, 58˚C 30 s, 72˚C 60 s for 35 cycles using 1 mM MgCl2 | 198 |