| 10× PCR buffer | 2.5 |
| 25 mM MgCl2 | 1.0 |
| 5 pMol forward primer | 1.0 |
| 5 pMol reverse primer | 1.0 |
| DMSO | 1.0 |
| 2.5 Mm DNTPs | 2.0 |
| Taq 5 u/ul | 0.1 |
| 10 ng/µl DNA | 3.0 |
| H2O | 13.4 |
| TOTAL | 25 µL |