|
| Water wash | 2 minutes |
| Differentiation | 1% acid alcohol | 3 dips |
|
| Water wash | 5 minutes |
| Bluing | Scott’s tap water | 15 minutes |
|
| Water wash | 3 minutes |
| Cytoplasmic staining | 1% Eosin | 20 seconds |
| Dehydration | 90% Alcohol | 30 seconds |
|
| 70% Alcohol | 30 seconds |
| Clearing | Xylene I | 5 minutes |
|
| Xylene II | 5 minutes |
| Mounting | DPX mountant |
|