| ACTIVITY | REAGENT | DURATION |
| Deparaffinizationand rehydration | Xylene I | 10 minutes |
|
| Xylene II | 10 minutes |
|
| 100% Alcohol | 5 minutes |
|
| 90% Alcohol | 5 minutes |
|
| Water wash | 5 minutes |
| Nuclear staining | Harris hematoxylin | 20 minutes |