ACTIVITY | REAGENT | DURATION |
Deparaffinization and rehydration | Xylene I | 10 minutes |
| Xylene II | 10 minutes |
| 100% Ethanol | 5 minutes |
| 90% Alcohol | 5 minutes |
| Water wash | 5 minutes |
Nuclear staining | Harri’s hematoxylin | 20 minutes |
| Water wash | 2 minutes |
Differentiation | 1% acid alcohol | 3 dips |
| Water wash | 5 minutes |
Bluing | Scott’s tap water | 10 minutes |
| Water wash | 5 minutes |
Cytoplasmic staining | Teak extracts (group 1) | 15 minutes |
| Teak extracts (group 2) | 10 minutes |
| Teak extracts (group 3) | 5 minutes |
| Water wash | 1 minutes |
Dehydration | Room temperature | 30 minutes |
Clearing | Xylene I | 5 minutes |
| Xylene II | 5 minutes |
Mounting | DPX mountant |
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