| ACTIVITY | REAGENT | DURATION |
| Deparaffinization and rehydration | Xylene I | 10 minutes |
|
| Xylene II | 10 minutes |
|
| 100% Ethanol | 5 minutes |
|
| 90% Alcohol | 5 minutes |
|
| Water wash | 5 minutes |
| Nuclear staining | Harri’s hematoxylin | 20 minutes |
|
| Water wash | 2 minutes |
| Differentiation | 1% acid alcohol | 3 dips |
|
| Water wash | 5 minutes |
| Bluing | Scott’s tap water | 10 minutes |
|
| Water wash | 5 minutes |
| Cytoplasmic staining | Teak extracts (group 1) | 15 minutes |
|
| Teak extracts (group 2) | 10 minutes |
|
| Teak extracts (group 3) | 5 minutes |
|
| Water wash | 1 minutes |
| Dehydration | Room temperature | 30 minutes |
| Clearing | Xylene I | 5 minutes |
|
| Xylene II | 5 minutes |
| Mounting | DPX mountant |
|