| Primer Name | Sequence(5’-3’) | Description |
| TGR-F | GCTCTAGAATGTCAGGAAATCCACTGGATGC | Generation for pTRV2-GFP-RrGT1 |
| TGR-R | CCCTCGAGCTGCAGTGGTAATGAGAGGGAG |
|
| TRV1-F | TTACAGGTTATTTGGGCTAG | RT-PCR for TRV1 |
| TRV1-R | CCGGGTTCAATTCCTTATC |
|
| TRV2-F | TGGGAGATGATACGCTGTT | Confirmation for recombinant silencing vector and RT-PCR for TRV2 RT-PCR for GFP |
| TRV2-R | CCTAAAACTTCAGACACG | |
| GFP-F | ATGGTGAGCAAGGGCGAGGA | |
| GFP-R | CTTGTACAGCTCGTCCATGCC | |
| RrGAPDH-F | TTCTGCCTGCTCTCAATG | RT-PCR and qRT-PCR for RrGAPDH |
| RrGAPDH-R | TGCCTTCTTCTCAAGTCTG |
|
| qRrGT1-F | GTATTTGCCAACACACTGAGTAA | qRT-PCR for RrGT1 |
| qRrGT1-R | CTGCAGTGGTAATGAGAGGGAG |
|