| Groups Manipulation | Pain Stimulation | AANs Ablation or Gene Silence | GA Administration |
| Group 1 | Yes | No | No |
| Group 2 | Yes | No | Yes |
| Group 3 | Yes | SON, paraSON, VLPO | Yes |
| Group 4 | Yes | SON, paraSON | Yes |
| Group 5 | Yes | VLPO | Yes |