| Genes | GenBank accession No. | Primer sequence (5’-3’) | Product size, bp |
| GAPDH | NM_001082253 | F: TGCCACCCACTCCTCTACCTTCG R: CCGGTGGTTTGAGGGCTCTTACT | 163 |
| NPY | AB469827 | F: CCTCATCACCAGGCAGAGAT R: ATTTCGTTTCCCATCACCAC | 137 |
| AgRP | XM_002711615 | F: GCTACTGCCGCTTCTTCAAC R: CCATTCTTTATTGGCGTTCC | 133 |
| POMC | XM_008254814 | F: GCCTGGAAGATGCTGAGGT R: CTCCTGACACTGGCTGCTCT | 102 |
| CART | XM_008274526 | F: AGGAGCCAGGATTGGGAAG R: CTGATGGAAGAGCGTGGAAG | 101 |
| PYY | XM_002719177 | F: CTGAACCGCTACTACGCCTC R: GTCTTCACCACGGGTAGGC | 166 |
| CRH | NM_001199007 | F: CATCTCCCTGGATCTCACCT R: CCATCAGTTTCCTGTTGCTG | 101 |
| MC4R | HF970577 | F: GGATACGGACGCACAGAGTT R: AATGGAGGCAAGCAAGGAG | 82 |
| CCK | XM_002713069 | F:AGCAACCTCCTGACCTTACG R:GGCACTCACTGGACGATTTA | 140 |