Parameters

Explants tested

Reps

DsRed expressing explants (t = 10 dac)

Agro infiltration methods

Dipping 10 min

25

3

5 ± 3^c

Vacuum 5 min

25

3

8 ± 1^bc

Vacuum 10 min

25

3

17 ± 2^a

Vacuum 15 min

25

3

11 ± 4^ab

Density of bacterial culture (OD₆₀₀)

0.25

25

3

8 ± 3^b

0.50

25

3

16 ± 2^a

0.75

25

3

15 ± 1^a

1.0

25

3

17 ± 2^a

Duration of co-cultivation period (days)

1

25

3

5 ± 2^c

2

25

3

11 ± 1^b

3

25

3

18 ± 2^a

4

25

3

17 ± 3^a

Transformation enhancer/acetosyringone (μM)

0

25

3

12 ± 2^b

50

25

3

14 ± 2^b

100

25

3

22 ± 1^a

150

25

3

21 ± 3^a