Run

CF

FF

Time

Temperature

IS

Proteolytic activity (U/ml)

Experimental

Predicted

1

10

10

48

33.5

6

672

624

2

40

10

48

33.5

6

2372

2071

3

10

30

48

33.5

6

1218

1451

4

40

30

48

33.5

6

3745

2898

5

25

20

24

30

6

1290

1608

6

25

20

72

30

6

2736

2671

7

25

20

24

37

6

1554

1601

8

25

20

72

37

6

1500

1164

9

25

10

48

33.5

2

1390

1348

10

25

30

48

33.5

2

1936

2174

11

25

10

48

33.5

10

1300

1348

12

25

30

48

33.5

10

2290

2174

13

10

20

24

33.5

6

1854

1347

14

40

20

24

33.5

6

1654

1862

15

10

20

72

33.5

6

1090

728

16

40

20

72

33.5

6

2754

3107

17

25

20

48

30

2

1909

2139

18

25

20

48

37

2

1354

1383

19

25

20

48

30

10

2081

2139

20

25

20

48

37

10

918

1383

21

25

10

24

33.5

6

954

1191

22

25

30

24

33.5

6

1654

2018

23

25

10

72

33.5

6

1272

1504

24

25

30

72

33.5

6

2500

2331

25

10

20

48

30

6

1190

1416

26

40

20

48

30

6

2500

2863

27

10

20

48

37

6

900

659

28

40

20

48

37

6

2509

2106

29

25

20

24

33.5

2

2290

1605

30

25

20

72

33.5

2

2018

1917

31

25

20

24

33.5

10

1845

1605

32

25

20

72

33.5

10

1727

1917

33

10

20

48

33.5

2

1036

1038

34

40

20

48

33.5

2

3127

2484

35

10

20

48

33.5

10

1263

1038

36

40

20

48

33.5

10

2136

2484

37

25

10

48

30

6

2054

1726

38

25

30

48

30

6

2918

2553

39

25

10

48

37

6

763

970

40

25

30

48

37

6

1127

1796

41

25

20

48

33.5

6

1681

1761

42

25

20

48

33.5

6

2000

1761

43

25

20

48

33.5

6

1563

1761

44

25

20

48

33.5

6

1254

1761

45

25

20

48

33.5

6

1672

1761

46

25

20

48

33.5

6

1436

1761