| Synthesis process |
Step 1 | Preparation of button mushroom extract: · Grind 50 g of button mushroom · Mix with 100 mL of distilled water · Filter all · Keep in the refrigerator |
Step 2 | Preparation of the tyrosine solution: · Measure 0.1 g of tyrosine powder · Mix with 100 mL of distilled water · Heat while stirring until the powder dissolves · Keep in the refrigerator |
Step 3 | Both reagents mixture: · In test tubes, mix 4 mL potato extract and 2 mL tyrosine · Immerse the tubes in a calibrated water bath at 37˚C · Wait an hour and then observe the color |