Synthesis process

Step 1

Preparation of button mushroom extract:

· Grind 50 g of button mushroom

· Mix with 100 mL of distilled water

· Filter all

· Keep in the refrigerator

Step 2

Preparation of the tyrosine solution:

· Measure 0.1 g of tyrosine powder

· Mix with 100 mL of distilled water

· Heat while stirring until the powder dissolves

· Keep in the refrigerator

Step 3

Both reagents mixture:

· In test tubes, mix 4 mL potato extract and 2 mL tyrosine

· Immerse the tubes in a calibrated water bath at 37˚C

· Wait an hour and then observe the color