Types of PCR	Primers	Sequences
Nested PCR for diagnostic on DNA
HLA	GH26 Forward	5’-GTGCTGCAGGTGTAAACT-3’
HLA	GH27 Reverse	5’-CACGGATCCGGT-3’
Gag	GAG1 Forward	5’-GGTACATCAGGCCATATCACC-3’
Gag	GAG4 Reverse	5’-ACCGGTCTACATAGTCTC-3’
Pol	POLITG1 Forward	5’-CCCTACAATCCCCAAAGTCAAGG-3’
Pol	POLITG4 Reverse	5’-TACTGCCCCTTCACCTTTCCA-3’
Env	ENV1 Forward	5’-GAGGATATAATCAGTTTATGG-3’
Env	ENV4 Reverse	5’-AATTCCATGTGTACATTGTACTG-3’
Nested gag	GAG2 Forward	5’-GAGGAAGCTGCAGAATGGG-3’
Nested gag	GAG3 Reverse	5’-GGTCCTTGTCTTATGTCC-3’
Nested pol	POLITG2 Forward	5’-TAAGACAGCAGACAAATGGCAG-3’
Nested pol	POLITG3 Reverse	5’-GCTGTCCCTGTAATAAACCCG-3’
Nested env	ENV2 Forward	5’-GATCAAAGCCTAAAGCCATG-3’
Nested env	ENV3 Reverse	5’-CAATAATGTATGGGAATTGG-3’
Quantitative PCR (qPCR) for Viral Load on RNA
qPCR	HIV1MG Forward	5’-GCCTCAATAAAGCTTGCCTTGA-3’
	HIV1MG Reverse	5’-GGCGCCACTGCTAGAGATTTT-3’
	HIV1MG Probe	FAM-5’-AAGTAGTGTGTGCCCGTCTGTTRTKTGACT-3’-BHQ1
PCR for Sequencing
RT-PCR Prot	5’ prot 1	5’-TAATTTTTTAGGGAAGATCTGGCCTTCC-3’
RT-PCR Prot	3’ prot 1	5’-GCAAATACTGGAGTATTGTATGGATTTTCAGG-3’
Nested PCR Prot	5’ prot 2	5’-TCAGAGCAGACCAGAGCCAACAGCCCCA-3’
Nested PCR Prot	3’ prot 2	5’-AATGCTTTTATTTTTTCTTCTGTCAATGGC-3’
RT-PCR RT	MJ3	5’-AGTAGGACCTACACCTGTCA-3’
RT-PCR RT	MJ4	5’-CTGTTAGTGCTTTGGTTCCTCT-3’
Nested PCR RT	A(35)	5’-TTGGTTGCACTTTAAATTTTCCCATTAGTCCTATT-3’
Nested PCR RT	NE1(35)	5’-CCTACTAACTTCTGTATGTCATTGACAGTCCAGCT-3’
RT-PCR Alt Prot	5’ eprB	5’-AGAGCTTCAGGTTTGGGG-3’
RT-PCR Alt Prot	3’ eprB	5’-GCCATCCATTCCTGGCTT-3’
Nested PCR Atl Prot	5’ prB	5’-GAAGCAGGAGCCGATAGACA-3’
Nested PCR Atl Prot	3’ prB	5’-ACTGGTACAGTTTCAATAGG-3’
RT-PCR Atl RT	RT1	5’-CCAAAAGTTAAACAATGGCCATTGACAGA-3’
RT-PCR Atl RT	RT4	5’-AGTTCATAACCCATCCAAAG-3’
Nested PCR Alt RT	RT18	5’-GGAAACCAAAAATGATAGGGGGAATTGGAGG-3’
Nested PCR Alt RT	RT21	5’-CTGTATTTCTGCTATTAAGTCTTTTGATGGG-3’