| Improvement | Effect |
| Decrease of Pump A rate to 3 mL・min−1 | Eliminate air from surroundings into syringe A |
| New loading procedure for internal DNA control | Improved mixing of internal control (IC) DNA and Milli-Q water |
| Increased total volume of diluted internal DNA control to sample volume | Improved loading of IC DNA |
| New dye loading procedure. Dead space in Pump B valve used as dye dosing volume | Accurate dye dosing |
| Selection of incubation conditions of 13 min at 60˚C | Improved staining of cells with SYBR Green I |
| No air is moved through flow cell | Eliminate introduction of air bubbles in flow cell |
| Flow direction of flow cell changed | Remove potential air bubbles in flow cell |
| New flow rate through flow cell | Optimal flow rate for maximum reading |
| Increased rate of dispensing and aspirating with Pump A for all non-critical steps | Minimize total AMS measurement cycle time to 75 min |
| Incorporated data export function | For automatic plot of raw and calibrated readings on graph in Excel |