Part

Prototype AMS

Pre-industrial AMS

Unit layout

Single box

Three modules: hardware, consumables, and battery

Internal DNA

control

N/A

Synthetic DNA in

ethyleneiaminetetraacetic acid

(EDTA) and ascorbate buffer

Sample staining

40 min at ambient temperature

13 min at 60˚C

Flow cell

Commercially available

flow cell

Custom-built flow cell with minimal volume and large numerical aperture

Measurement

Sample is static when

illuminated

Sample flows slowly when illuminated to avoid bleaching of dye

Dye dispensing

Fixed volume

Dedicated syringe pump for dye

Lower limit of

field detection

~105

Low limit of detection, LLOD

− AMS 1: 1.9 × 104; AMS 2: 1.2 × 104

Low limit of quantification, LLOQ

− AMS 1: 5.0 × 104; AMS 2: 3.2 × 104

Cleaning

NaOH

NaOH and oxalic acid

Power

AC mains

Battery, charged either by AC mains or solar panels

Remote communication

Team Viewer through

Saudi Aramco intranet when AMS is on

Permanent RS-485 connection. Data retrieval and AMS control through dedicated software on remote computer