| Item | [18F]FLT | [18F]FMISO |
| Reactor Temperature− | 170˚C | 170˚C |
| Reactor Flow Rate | 150 μl/min | 150 μL/min |
| Precursor Concentration in CH3CN | 20 mg/mL | 5 mg/mL |
| Acid Hydrolysis | 2 N HCl/2 mL 95˚C (6 min) | 1 N HCl/0.5 mL 100˚C (5 min) |
| Neutralization | 3 N NaOAc/2 mL | 1 N NAOH/1 mL |
| Semi-Preparative HPLC | 8% EtOH in PBS | 5% EtOH in H2O |
| Flow Rate | 5 mL/min | 5 mL/min |
| Time of Peak Collection | ~12 min | ~9 min |