| 2 mM dNTP | 1.5 ul |
| 10 × KOD buffer | 1.5 ul |
| MgSO4 | 0.6 ul |
| DMSO | 1.5 ul |
| Primer 5-HTT-F: (10 uM) | 1 ul |
| Primer 5-HTT-R: (10 uM) | 1 ul |
| template | 1 ul |
| Blend Taq-Plus | 0.5 ul |
| ddH2O | 6.4 ul |
| Total | 15 ul |