| Matrix stiffness | 40% Acrylamide (μl) | 2% Bis-acrylamide (μl) | 1 M HEPES (μl) | ddH2O (μl) | Total (μl) |
| 1 kPa | 30.00 | 5.00 | 2.00 | 163.00 | 200.00 |
| 40 kPa | 73.60 | 30.20 | 2.00 | 94.20 | 200.00 |