70% ethanol pre-treatment (5min treatment)

Amount of persulfuric acid (completed to 25 ml with deionized water, 10 min treatment)

2 V

70% ethanol final treatment

A

1 mL

+

B

2.5 mL

+

C

5 mL

+

D

10 mL

+

Aeth

+

1 mL

+

Beth

+

2.5 mL

+

Ceth

+

5 mL

+

Deth

+

10 mL

+