| 70% ethanol pre-treatment (5min treatment) | Amount of persulfuric acid (completed to 25 ml with deionized water, 10 min treatment) 2 V | 70% ethanol final treatment |
A | − | 1 mL | + |
B | − | 2.5 mL | + |
C | − | 5 mL | + |
D | − | 10 mL | + |
Aeth | + | 1 mL | + |
Beth | + | 2.5 mL | + |
Ceth | + | 5 mL | + |
Deth | + | 10 mL | + |