| Name | Sequence | Annealing temperature, ˚C | Expected size, bp | Gene product |
| ituC-F | TGCCATTATTGTCTACGGAG | 50 | 270 | acyl-protein synthetase ItuC |
| ituC-R | ATAAATCATACAGCCGAC | 43 | ||
| bmyB-F | ACGGCAGGTTTTGATTTTT | 45 | 290 | bacillomycin L synthetase BmyB |
| bmyB-R | CGTTCCTTATCTCCGGA | 47 | ||
| bacA-F | CATTTCCAATTTTACTCTTC | 44 | 410 | bacilysin biosynthesis protein BacA |
| bacA-R | TACTTTTGCCGTGCAAGCTC | 52 | ||
| srfA-F | AACGGGGAGCCTGTTCAATA | 52 | 420 | surfactin synthase subunit 1 SrfAA |
| srfA-R | ACAAGTTCAGGCACCGATTC | 52 | ||
| fenD-F | AAAGGTGTGTGGAATTGATG | 48 | 430 | fengycin synthetase FenD |
| fenD-R | GCTGTCTCCTCTATCAAAAA | 48 |